PD-L1 Antibody / B7-H1 / CD274

SKU: orb2644677

Description

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancers ability to evade the immune system.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant PDL1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG2a, kappa
• Clone No.: rPDL1/4773
• Immunogen: A portion of amino acids 39-191 from the human protein was used as the immunogen for the recombinant PDL1 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant PDL1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human placenta with recombinant PDL1 antibody (clone rPDL1/4773). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin with recombinant PDL1 antibody (clone rPDL1/4773). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PDL1 antibody (clone rPDL1/4773) as confirmation of integrity and purity.

Western blot testing of human A549 cell lysate with recombinant PDL1 antibody (clone rPDL1/4773).

Western blot testing of mouse and human cell lysates with recombinant PDL1 antibody (clone rPDL1/4773).

Quick Database Links

UniProt

Q9NZQ7