PD-L1 / CD274 Extracellular domain (IHC prefer)

SKU: orb1294267

Description

Rabbit polyclonal antibody to PD L1

Research Area

,Product Categories/Antibodies,Immune Checkpoints / Cancer Biomarker

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: Western Blot & Immunoprecipitation: Reported in the literature, not verified in house. Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:100
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human PD-L1 / CD274
• Target: PD-L1 / CD274 Extracellular domain (IHC prefer)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 25% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Customer Feedback: Cell: MDA- MB- 231; 1Ab: orb1294267 1-500; 2Ab: anti Rabbit IgG (Light chain) 1-3000.

Immunohistochemical analysis of paraffin embedded Human colon cancer tissue labeling PD-L1/CD274 with orb1294267 at 1/100.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1/CD274 with orb1294267 at 1/150.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. stained with orb1294267 Anti-PD-L1/CD274 antibody (red) at 1:200 and 4°C.

Quick Database Links

Gene Symbol

PD-L1 / CD274 Extracellular domain (IHC prefer)