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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Canine |
| Biological Activity | PD-L1 Protein, Canine, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.5 kDa and the accession number is A0A8C0MRV9. |
| Tag | C-hFc |
| Expression Region | A DNA sequence encoding the canine CD274 (NP_001278901.1) (Met1-Arg236) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 19 |
| MW | 51.5 kDa (predicted); 77.93 and 149.69 kDa (reducing condition, due to glycosylation) |
| Purity | SDS-PAGE: 93.5%; SEC-HPLC: 94.6% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Canine PD-L1 Protein, hFc Tag [orb2321231]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.1 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg PD-L1 Protein, Canine, Recombinant (hFc), Biotinylated [orb1953756]
98.00%
51.5 kDa (predicted); 77 and 145.5 kDa (reducing condition, due to glycosylation)
100 μg, 20 μg
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Protocol Information
Protein Handling and Storage Guide
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