PDGFAA + PDGFBB Rabbit Polyclonal Antibody

SKU: orb158129

Description

PDGFAA + PDGFBB Rabbit Polyclonal Antibody

Research Area

Angiogenesis and Vasculogenesis, Varscular System, Cardiovascular Research, Cardiovascuolar, Hypoxia, Inflammatory Mediators, Inflammation, Platelet-derived growth factor

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDGFA and PDGFB (81-180/241aa)
• Target: PDGFB
• Molecular Weight: 12 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Becaplermin; c sis; c-sis; FLJ12858; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B; PDGF B chain; PDGF subunit B; PDGF-2; Pdgfb; PDGFB_HUMAN; Platelet derived growth factor A chain precursor; Platelet derived growth factor alpha polypeptide; Platelet derived growth factor B chain precursor; Platelet derived growth factor beta polypeptide(simian sarcoma viral(v-sis) oncogene homolog); Platelet derived growth factor beta polypeptide; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor subunit B; Platelet-derived growth factor, beta polypeptide(oncogene SIS); Proto-oncogene c-Sis; SIS; SSV; v sis platelet derived growth factor beta polypeptide(simian sarcoma viral oncogene homolog).

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDGFAA + PDGFBB) Polyclonal Antibody, Unconjugated (orb158129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDGFAA + PDGFBB) Polyclonal Antibody, Unconjugated (orb158129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PDGFB

UniProt

P01127