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Description
Research Area
,Apoptosis,Cancer,Cell Cycle,Immunology
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human PDL1 /CD274 Protein at 5μg/mL (100 μl/well),can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 0. 16-2. 4μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2μg/ml (100μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 0. 05-1μg/ml. Measured by its ability to inhibit antiCD3 antibody induced IL2 secretion in human T lymphocytes. The ED50 for this effect is typically 0. 6-5μg/ml. |
| Target | CD274 |
| Tag | His Tag |
| MW | 26 kDa |
| Purity | > 98% as determined by SEC-HPLC and SDS-PAGE. |
| Protein Sequence | Phe 19 - Arg 238 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_054862.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
Available Sizes
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