You have no items in your shopping cart.
Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Strep Tag at 5 ug/mL (100 ul/well) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 2-60 ng/mL. |
| Target | CD274 |
| Tag | C-Twin Strep Tag |
| MW | 28.2 kDa |
| Purity | > 95% as determined by SDS-PAGE. |
| Protein Sequence | Phe 19 - Arg 238 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−PD-L1 Monoclonal Antibody [orb688864]
ELISA, FC, IF, IHC, WB
Human
Mouse
Monoclonal
Unconjugated
50 μl, 100 μlRecombinant human PD-L1 protein, C-His (HEK293) [orb1516352]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result. kDa
100 μgRecombinant human PD-L1 protein, C-mFc (HEK293) [orb1516351]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. kDa
100 μgHuman PD-L1(19-132) Protein, hFc Tag [orb2321289]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.2 kDa after removal of the signal peptide. The apparent molecular mass of PD-L1(19-132)-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgRecombinant human PD-L1 protein, C-His-Avi (HEK293), Biotin conjugated [orb2328736]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 35-48 kDa based on Tris-Bis PAGE result. kDa
20 μg

Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_054862.1 |
|---|























