PDX1 Antibody (T11)

SKU: orb1931774

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cancer;Metabolism;Signal Transduction;Mitochondrion

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15204
• Target: This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1.
• Molecular Weight: 54122 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate dehydrogenase protein X component, mitochondrial, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, proX, PDHX, PDX1

Fluorescent confocal image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-PDX1 Antibody (T11) in NCI-H460 cell line lysates (35 ug/lane).PDX1-T11 (arrow) was detected using the purified Pab.

Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2).

Quick Database Links

Gene Symbol

This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1.

UniProt

O00330

RefSeq (Protein):NP_003468.2, NP_001159630.1, NP_001128496.1