PECAM-1 Antibody [C31.7]

SKU: orb1252720

This product can be custom made

Description

Research Area

,Cancer,Immunology,Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	FC, IF, IHC, WB
Reactivity	Human, Primate, Rabbit
Application Notes	Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone No.	C31.7
Immunogen	Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody.
Target	PECAM1
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration	0.2 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

Quality Guarantee

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