PECAM1 monoclonal antibody (M01), clone 1D2-1A5

SKU: orb2292425

Description

Mouse monoclonal antibody raised against a full length recombinant PECAM1.

Images & Validation

• Tested Applications: ELISA, IHC-P, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone No.: 1D2-1A5
• Immunogen: PECAM1 (AAH22512, 29 a.a. ~ 738 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: ENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western Blot detection against Immunogen (103.95 KDa).

Detection limit for recombinant GST tagged PECAM1 is 0.1 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 5 ug/ml]

Immunoprecipitation of PECAM1 transfected lysate using anti-PECAM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PECAM1 MaxPab rabbit polyclonal antibody.

PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.

Western Blot analysis of PECAM1 expression in transfected 293T cell line by PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Lane 1: PECAM1 transfected lysate (82.4 KDa). Lane 2: Non-transfected lysate.

Quick Database Links

RefSeq:AAH22512