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Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | PEDF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is A0A140VKF3. |
| Tag | C-His |
| Expression Region | A DNA sequence encoding the human SERPINF1 (NP_002606.3) (Met 1-Pro 418) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 20 |
| MW | 45.8 kDa (predicted); 45.8 kDa (reducing conditions) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Human PEDF Protein, His Tag [orb2321431]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.2 kDa after removal of the signal peptide. The apparent molecular mass of PEDF-His is approximately 35-55 kDa due to glycosylation.
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> 95% as determined by SDS-PAGE
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Protocol Information
PEDF Protein, Human, Recombinant (His) (orb1958306)
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