PHF10 Antibody

SKU: orb2634934

Description

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.

Images & Validation

• Tested Applications: ELISA, FACS, IF, WB
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PHF10 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: PCRP-PHF10-2A10
• Immunogen: Recombinant full-length human PHF10 protein was used as the immunogen for the PHF10 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This PHF10 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells using PHF10 antibody (green, clone PCRP-PHF10-2A10) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with PHF10 antibody (blue, clone PCRP-PHF10-2A10) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free PHF10 antibody (clone PCRP-PHF10-2A10) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PHF10 antibody (clone PCRP-PHF10-2A10). These results demonstrate the foremost specificity of the PCRP-PHF10-2A10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

Q8WUB8