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Description
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:4000; IHC: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | v-akt murine thymoma viral oncogene homolog 1 |
| Target | Phospho-Akt(Ser473) |
| Molecular Weight | 60KD |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT, AKT1, AKT1-phospho-S473, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
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Quick Database Links
Gene Symbol
Phospho-Akt(Ser473)
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)























