Phospho-CDK7(T170) Antibody

SKU: orb527214

Description

Rabbit polyclonal antibody to CDK7 (phospho-T170)

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Cancer/DNA Damage,Epigenetics,Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Cell Biology;Signal Transduction;Phospho Antibodies;Catalog Updated

Images & Validation

• Tested Applications: DOT, IHC-P, WB
• Dilution Range: WB: 1:1000, DOT: 1:500, IHC-P: 1:50-100
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB07518
• Immunogen: Synthetic Peptide
• Target: CDK7
• Molecular Weight: 39038
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase 7, 39 kDa protein kinase, p39 Mo15, CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, TFIIH basal transcription factor complex kinase subunit, CDK7, CAK, CAK1, CDKN7, MO15, STK1

Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with λ phosphatase, using 459667101 (upper) or Tubulin (lower).

Western blot analysis of extracts from Hela and MCF-7 cells, untreated or lamda phosphatase-treated, using Phospho-CDK7 (T170) Antibody (upper) or Beta-actin (lower).

Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

CDK7

UniProt

P50613

RefSeq (Protein):NP_001790.1