Phospho-eNOS (Ser1177) Rabbit Polyclonal Antibody

SKU: orb6519

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Description

Research Area

Cardiovascular Research, Hypoxia, Nitric oxide (NO), Neuroscience, Oxidative Stress

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg /Test, ELISA=1:2000-10000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser1177 TQ(p-S)FS
Target	NOS3
Molecular Weight	133 kDa
Purification	affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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eNOS(phospho S1177); eNOS(phospho S1177); p-eNOS(phospho S1177); eNOS(phospho-Ser1177); cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3(endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.

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Phospho-eNOS (Ser1177) Rabbit Polyclonal Antibody

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human HUVEC treated with 100 µM Pervanadate (PV) for 10 minutes, Primary: Anti-Phospho-eNOS (Ser1177) (orb6519) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 133 kDa, Observed band size: 135 kDa.

The blue histogram is unstained cells (HepG 2 cells). The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-Phospho-eNOS (Ser1177)/FITC Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg in 100 µl 1X PBS containing 0.5% BSA.