Phospho-HIST1H3B3(S10) Antibody

SKU: orb34452

Description

Rabbit polyclonal to HIST1H3B3 (phospho-Ser10)

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Antibody Collections;Phospho Antibodies

Images & Validation

• Tested Applications: DOT, IF, IHC-P, WB
• Dilution Range: WB: 1:2000, DOT: 1:500, IHC-P: 1:50-100
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB2673; RB2674
• Immunogen: Synthetic Peptide
• Target: H3C1
• Molecular Weight: 15404
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Histone H3.1 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3/c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody

Western blot analysis of lysates from Hela cell line, untreated or treated with nocodazole (1ug/ml, 18h), using Phospho-HIST1 h3B3 (S10) Antibody (upper) or Beta-actin (lower).

Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-HIST1 h3B3 (S10) Antibody (upper) or Beta-actin (lower).

Dot blot analysis of anti-Phospho-HIST1 h3B3-S10 Phospho-specific Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela cells labeling HIST1 h3A at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

H3C1

UniProt

P68431

RefSeq (Protein):NP_003521.2, NP_003520.1, NP_003525.1, NP_003527.1, NP_003522.1, NP_003524.1, NP_003526.1, NP_003528.1, NP_066298.1, NP_003523.1