phospho Histone H2A.X (Ser139), monoclonal antibody

SKU: orb1973161

This product can be custom made

Description

Research Area

,Cell cycle / DNA damage

Images & Validation

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Item 1 of 3

Tested Applications	IF, WB
Dilution Range	Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:200 Immunohistochemistry (Paraffin) 1:50 – 1:100
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Target	phospho Histone H2A.X (Ser139), monoclonal antibody
Conjugation	Unconjugated

Storage & Handling

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Storage	Long time storage is recommended at -20°C Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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phospho Histone H2A.X (Ser139), monoclonal antibody at 1/500 dilution. Lysates/at 35 μg per lane. NC blot was then blocked for an hour before being incubated with orb1973161 overnight at 4°C.

Immunofluorescence of cell. phospho Histone H2A.X (Ser139), monoclonal antibody (orb1973161): 1-200.

Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue.

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phospho Histone H2A.X (Ser139), monoclonal antibody

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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