Phospho-HSP27 (Ser82) (CB2) rabbit mAb

SKU: orb1946056

Description

Phospho-HSP27 (Ser82) (CB2) rabbit mAb

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Clone No.: HSP27S82-CB2
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human phospho Hsp27
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HSP28

Western blot analysis of HeLa cell extract, untreated or treated with UV plus TPA using Phospho-HSP27 (Ser82) antibody HSP27S82-CB2 at 5 ng/mL. Cat. # orb1946056

Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) using Phospho-HSP27-CB2 (Ser82) antibody HSP27S82-CB2 at 1.0 ng/mL. Cat. #orb1946056.

Peptide blocking flow cytometric analysis of HeLa cells unstained and untreated as negative control (light blue) or stained and untreated (red) or treated with UV + plus TPA (green) or untreated and blocked with phospho-peptide (black) or UV plus TPA blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV plus TPA and blocked with non-phospho peptide (purple) using Phospho-HSP27 (Ser82) antibody HSP27S82-CB2 at 10 μg/mL, Cat#orb1946056.

Quick Database Links

UniProt

P04792