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Phospho-JUN Antibody (pT91/93)

SKU: orb2642509

Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [RefSeq]

Images & Validation

Tested ApplicationsFACS, IF, IHC-P, WB
Dilution RangeFlow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Optimal dilution of the phospho-c-Jun antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.C-J 4C4/1
ImmunogenThe amino acids surrounding phosphorylated T91 and T93 were used as the immunogen for the phospho-c-Jun antibody.
PurityProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
Expiration Date12 months from date of receipt.
Hazard InformationThis phospho-c-Jun antibody is available for research use only.
DisclaimerFor research use only

Similar Products

  • Phospho-JUN Antibody (pT91/93) [orb606906]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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100 μg
$ 780.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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