Phospho-MEK1/2 (Ser221) (D3) rabbit mAb

SKU: orb1946141

Description

Phospho-MEK1/2 (Ser221) (D3) rabbit mAb

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Clone No.: MEK12S221-D3
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser221 of human phospho MEK1/2.
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 1/2, MAPK/ERK kinase 1, MAPKK1, MAPKK2, MAP2K1, MAP2K2, PRKMK1, PRKMK2

Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Western blot analysis of C2C12 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate (green) or imatinib and blocked with phospho peptide (gold) or pervanadate and blocked with phospho peptide (black) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-MEK1/2 (S221) antibody MEK12S221-D3 at 0.1 μg/mL. Cat. #orb1946141.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-MEK1/2 (S221) antibody MEK12S221-D3 0.1 μg/mL. Cat. #orb1946141.

Western blot analysis of A549 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3 Cat. #orb1946141 or Company A antibody at 0.005 μg/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P36507, Q02750