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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S536 of human RelA |
| Target | Phospho-NF-kB p65 (S536) |
| Molecular Weight | 65 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Phospho-NF-kB p65/RelA-S536 polyclonal antibody [orb1678392]
WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl

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NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb1292536 (Phospho-NF-kB p65-S536 antibody) at dilution of 1:1000, treated by Calyculin A (100nM) at 37℃ for 30min after serum-starvation overnight.

Immunohistochemistry of paraffin-embedded rat brain tissue slide using orb1292536 at dilution of 1:100.
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Protocol Information
Phospho-NF-kB p65 (S536) Antibody (orb1292536)
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