Phospho-p53(S20) Antibody

SKU: orb1931243

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Anti-Wnt-Pathway (plus Lgr5) Antibodies;SARS-Human interaction partners;Necrosis;Necroptosis;Mitochondrion;Apoptosis signaling;Apoptosis;Host-virus interaction;Viral process;Primary Antibodies;Phospho Antibodies;Amyotrophic Lateral Sclerosis (ALS);Signal Transduction;Microbiology;Cell Biology;Cancer;Antibody Collections

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human
• Predicted Reactivity: Hamster, Porcine, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7710
• Target: This p53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S20 of human p53.
• Molecular Weight: 43653 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-hp53 Pab in A2058 cell line lysate. hp53 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

This p53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S20 of human p53.

UniProt

P04637

RefSeq (Protein):NP_000537.3, NP_00126, NP_001263624.1, NP_001263625.1, NP_001119584.1, NP_001119586.1, NP_001119587.1, NP_001119589.1, NP_001263627.1, NP_001263628.1, NP_001263626.1, NP_001119590.1, NP_001119588.1, NP_001119585.1