Phospho-PDHE1A(S232) Antibody

SKU: orb34991

Description

Rabbit polyclonal antibody to PDHE1A (phospho-Ser232) is the pyruvate dehydrogenase complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO. Genetic variation in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Antibody Collections;Cancer;Metabolism;Signal Transduction;Phospho Antibodies;Mitochondrion

Images & Validation

• Tested Applications: DOT, WB
• Dilution Range: DOT: 1:500
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB29473
• Immunogen: Synthetic Peptide
• Target: PDHA1
• Molecular Weight: 43296
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-PDHE1A antibody

Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).

Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).

Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6 ug per ml.

Quick Database Links

Gene Symbol

PDHA1

UniProt

P08559

RefSeq (Protein):NP_001166927.1, NP_001166926.1, NP_000275.1, NP_001166925.1