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Description
Research Area
Cancer Research, Cytoplasmic, Cell Biology, Hypoxia, Intracellular Signaling Proteins, Cell Signaling, SMAD Family
Images & Validation
−| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:100, Flow-Cyt=3μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423 CS(p-S)VS |
| Target | SMAD3 |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Smad3(phospho Ser423); Smad3(phospho S423); p-Smad3(Ser423); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD(mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.
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Quick Database Links
Gene Symbol
SMAD3
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry


















