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Featured
Description
Research Area
Cancer Research, Cytoplasmic, Cell Biology, Hypoxia, Intracellular Signaling Proteins, Cell Signaling, SMAD Family
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 3E8 |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser425 SV(p-S) |
| Target | SMAD3 |
| Molecular Weight | 47 kDa |
| Purification | affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SMAD3(phospho S425); Smad3(phospho Ser425); p-Smad3(Ser425); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD(mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.
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Quick Database Links
Gene Symbol
SMAD3
UniProt
UniProt Details
− No UniProt data available
Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence


