Piccolo Antibody: APC

SKU: orb152167

Description

Rabbit polyclonal to Picollo (APC). Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), IHC (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Full length Protein, BC001304.1
• Target: Piccolo
• Molecular Weight: 550kDa
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACZ antibody, Aczonin antibody, Brain-derived HLMN protein antibody, DKFZp779G1236 antibody, KIAA0559 antibody, Multidomain presynaptic cytomatrix protein antibody, PCLO antibody, PCLO_HUMAN antibody, Piccolo (presynaptic cytomatrix protein) antibody, Piccolo mouse homolog of Pico antibody, Presynaptic cytomatrix protein antibody, Protein piccolo antibody

Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Piccolo

UniProt

Q9Y6V0

NCBI Gene ID

27445

RefSeq (Protein):NP_055325.2