PIK3CA Antibody: APC

SKU: orb414117

Description

Rabbit polyclonal antibody against PIK3CA conjugated to APC

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Cancer | Tumor Biomarkers | Oncoproteins | Immunology | Innate Immunity | TLR Signaling

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000)
• Reactivity: Mouse
• Application Notes:
  A 1:1000 dilution of SPC-741 was sufficient for detection of PIK3CA in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human PIK3CA (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: PIK3CA
• Molecular Weight: 55.7 kDa
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PI 3 Kinase catalytic subunit alpha antibody, Phosphoinositide 3 kinase catalytic alpha polypeptide antibody, PI3 kinase p110 subunit alpha antibody, PtdIns 3 kinase p110 antibody, PtdIns 3 kinase regulatory subunit alpha antibody, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 antibody, GRB1 antibody, PtdIns 3 kinase subunit p110 alpha antibody, Phosphoinositide 3 kinase regulatory subunit 1 (alpha) antibody, PtdIns-3-kinase subunit p110-alpha antibody, PtdIns-3-kinase subunit alpha antibody, PtdIns 3 kinase regulatory subunit p85 alpha antibody, p110alpha antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody, Phosphatidylinositol 3 kinase regulatory 1 antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody, PIK3R1 antibody, EC:2.7.11.1 antibody, Phosphoinositide-3-kinase catalytic alpha polypeptide antibody, PI3K alpha antibody, PI3-kinase subunit alpha antibody, Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody, p110 alpha antibody, Phosphatidylinositol 3 kinase regulatory subunit alpha antibody, Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody, 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody, PI3K-alpha antibody, PI3 kinase subunit alpha antibody, MGC142163 antibody, EC:2.7.1.153 antibody, PI3 kinase regulatory subunit alpha antibody, p85 alpha antibody, PI3Kalpha antibody, Phosphoinositide 3 kinase regulatory subunit 1 antibody, MGC142161 antibody, Serine/threonine protein kinase PIK3CA (EC:2.7.11.1) antibody, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody, PI3K antibody, Phosphatidylinositol 3 kinase associated p 85 alpha antibody, p85 antibody, Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha antibody, Phosphatidylinositol 4, PI3K regulatory subunit alpha antibody, PI3 kinase subunit p85 alpha antibody, PIK3CA antibody, PtdIns 3 kinase subunit alpha antibody

Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.

Quick Database Links

Gene Symbol

PIK3CA

UniProt

P42336

NCBI Gene ID

5290

RefSeq (Protein):NP_006209.2