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PKM2 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:2000-1:16000; IP: 1:200-1:1000; IHC: 1:100-1:1000; IF: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | PKM2 pyruvate kinase, muscle |
| Target | PKM2 |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Hela cells were subjected to SDS PAGE followed by western blot with orb395451 (PKM2-specific antibody) at dilution of 1:1000.

Immunofluorescent analysis of HepG2 cells using orb395451 (PKM2-specific antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

IP Result of anti-PKM2-specific (IP: orb395451, 4 ug; Detection: orb395451 1:300) with HeLa cells lysate 2400 ug.

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using orb395451 (PKM2-specific Antibody) at dilution of 1:50.
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PKM2 Antibody (orb395451)
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