Plakophilin 1 Antibody

SKU: orb386128

Description

Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1). Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Developmental Biology

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 10B2
• Immunogen: A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the Plakophilin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Plakophilin 1 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q13835