Podocalyxin Antibody / PODXL

SKU: orb749747

Description

Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the Podocalyxin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: 2A4
• Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.
• Purity: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Podocalyxin antibody is available for research use only.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Podocalyxin antibody (2A4).

Formalin-fixed, paraffin-embedded human placenta stained with Podocalyxin antibody (2A4).

Quick Database Links

UniProt

O00592

NCBI Gene ID

5420