PPHLN1 Antibody (N-term)

SKU: orb163636

Description

Rabbit polyclonal antibody to PPHLN1 (N-term).

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Target: PPHLN1
• Molecular Weight: 52737
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti PPHLN1 antibody, anti Periphilin-1 antibody, anti Periphilin-1 antibody, anti Gastric cancer antigen Ga50 antibody

Immunofluorescent analysis of MRGX4 staining in A549 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of MRGX4 expression in U87MG (A), C6 (B), MCF7 (C) whole cell lysates.

Quick Database Links

Gene Symbol

PPHLN1

UniProt

Q8NEY8

RefSeq (Protein):NP_001137259.1, NP_001137260.1, NP_958847.1, NP_958848.1, NP_057572.5, NP_958846.1, NP_958923.1