PPIA Antibody (N-term)

SKU: orb1431251

Description

Rabbit polyclonal antibody to PPIA.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Infectious Diseases,Primary Antibodies;Immunology;Signal Transduction;Host-virus interaction;SARS-Human interaction partners

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human, Mouse
• Predicted Reactivity: Hamster, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20884
• Immunogen: Synthetic Peptide
• Target: PPIA
• Molecular Weight: 18012
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, PPIA, CYPA

All lanes: Anti-PPIA Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of widr cells using PPIA Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PPIA Antibody (N-term) in T47D cell line (lane 1) and mouse brain (lane 2), bladder (lane 3) tissue lysates (35 ug/lane). PPIA (arrow) was detected using the purified Pab. (2 ug/ml)

Quick Database Links

Gene Symbol

PPIA

UniProt

P62937

RefSeq (Protein):NP_066953.1