PRKCA Antibody [45F9]

SKU: orb1261306

Description

PRKCA Antibody [45F9]

Research Area

,Apoptosis,Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: [45F9]
• Immunogen: Recombinant protein of human PRKCA
• Target: PRKCA
• Molecular Weight: Observed: 77kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

Western blot analysis of extracts of various cell lines, using PRKCA antibody (orb1261306). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse brain using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse intestin using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of CRC cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of A549 cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of HeLa cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of MCF-7 cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of A549 cells using Acetyl-Histone H2A-K9 antibody (orb1255246).

Quick Database Links

Gene Symbol

PRKCA

UniProt

P17252