Proteinase K - Solid

SKU: orb532710

Description

Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5). Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. In presence of 0.5-1% SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Images & Validation

• Application Notes:
  Digestion of proteins during DNA and RNA preparation.

Key Properties

• Biological Activity: Specific activity: > 30 units/mg, DNase activity: not detectable, RNase activity: not detectable
• CAS Number: 39450-01-6
• MW: Theoretical MW: 28.9 kDa
• Purity: free of RNases, DNases and Exonucleases

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: powder; Color: white
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only