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Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b Kappa |
| Clone No. | 1A10-3G12 |
| Immunogen | PSMA7 (AAH04427, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Protein Sequence | MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Detection limit for recombinant GST tagged PSMA7 is approximately 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10 ug/ml]

Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5 ug/ml]

Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 5 ug/ml]

Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.

PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Western Blot analysis of PSMA7 expression in HeLa.

PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.

Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (53.02 KDa).
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Protocol Information
PSMA7 monoclonal antibody (M01), clone 1A10-3G12 (orb2292321)
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