PSMD2 (vPairTM) Antibodies

SKU: orb131868

Description

Rabbit polyclonal to PSMD2. PSMD2 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 nonidentical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD2 is one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, PSMD2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1.

Research Area

Product Categories/Products/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human, Mouse
• Application Notes:
  < strong>WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; < br>< strong>Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [PSMD2 (N) (R1723-1)] or the C-terminal [PSMD2 (C) (R1723-2)] region of human PSMD2.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or the C-terminal region of human PSMD2
• Molecular Weight: 100
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti 26S proteasome non-ATPase regulatory subunit 2 antibody pairs, anti 26S proteasome regulatory subunit RPN1 antibody pairs, anti 26S proteasome regulatory subunit S2 antibody pairs, anti 26S proteasome subunit p97 antibody pairs, anti Protein 55.11 antibody pairs, anti Tumor necrosis factor type 1 receptor-associated protein 2 antibody pairs, anti TRAP2 antibody pairs, anti MGC14274 antibody pairs, anti P97 antibody pairs, anti Rpn1 antibody pairs, anti S2 antibody pairs

Quick Database Links

UniProt

Q13200