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Pycard Antibody

SKU: orb1248435

Description

Pycard Antibody

Research Area

,Apoptosis,Apoptosis

Images & Validation

Tested ApplicationsELISA, WB
ReactivityMouse
Predicted ReactivityBovine, Canine, Human, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 21+24kDa band observed in Mouse Spleen lysates (calculated MW of 21.5kDa according to NP_075747.3). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DLTDKLVSYYLES
TargetPycard
Molecular WeightApprox 21 + 23kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Pycard, PYD and CARD domain containing, 9130417A21Rik, Asc, CARD5, TMS-1, TNS1, masc, PYD and CARD domain-containing protein, apoptosis-associated speck-like protein containing a CARD

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

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