Pyruvate Kinase (PKM2) Antibody (C-term)

SKU: orb1788119

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000, WB: 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 476-505 aa
• Target: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• Molecular Weight: 57937 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2

Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate (from left to right), using PKM2-N491. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

All lanes: Anti-PKM2 Antibody N491 at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: mouse brain lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: PC-12 whole cell lysates. Lane 5: Ramos whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57.9 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).

UniProt

P14618

RefSeq (Protein):NP_872271.1, NP_001193727.1, NP_872270.1, NP_001193726.1, NP_001193725.1, NP_001193728.1, NP_002645.3