R Cdk4 Antibody (C-term)

SKU: orb163624

Description

Rabbit polyclonal antibody to Rat Cdk4 (C-term).

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Target: Cdk4
• Molecular Weight: 33799
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti Cdk4 antibody, anti Cyclin-dependent kinase 4 antibody

Immunofluorescent analysis of CRK (pY221) staining in HuvEc Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of C/EBP alpha (pS21) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of C/EBP alpha (pS21) expression in rat lung (A) whole cell lysates.

Western blot analysis of CRK (pY221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates.

Quick Database Links

Gene Symbol

Cdk4

UniProt

P35426

RefSeq (Protein):NP_446045.1