Rabbit anti-ARID1B Recombinant Monoclonal Antibody [BLR291L]

SKU: orb1806439

Description

Rabbit anti-ARID1B Recombinant Monoclonal Antibody [BLR291L]

Research Area

,Primary Antibodies

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB: 1:1000 (3-8% Tris-acetate gel recommended), IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Immunogen: Between 1425 and 1475
• Target: ARID1B
• Conjugation: Unconjugated

Storage & Handling

• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSS1; MRD12; Osa homolog 2; P250R; hOsa2; ELD/OSA1; ELD (eyelid)/OSA protein; DAN15; OSA2; BRG1-binding protein hELD/OSA1; BRG1-binding protein ELD/OSA1; BRG1-associated factor 250b; BAF250B; AT-rich interactive domain-containing protein 1B; AT rich interactive domain 1B (SWI1-like); ARID domain-containing protein 1B; 6A3-5; BRIGHT

Detection of human ARID1B by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse ARID1B by western blot. Samples: Whole cell lysate (25 µg) from RenCa, EL4, A20, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1B/BAF250 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used for IP at 6 µl/mg lysate.

Detection of mouse ARID1B by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

ARID1B

UniProt

Q8NFD5