Rabbit anti-B7-H6 Recombinant Monoclonal Antibody [BLR271L]

SKU: orb1784569

This product can be custom made

Description

Research Area

,Primary Antibodies

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IP, WB
Dilution Range	WB: 1:1000, IP: 6 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Immunogen	Residues 25-262 (ECD)
Target	B7-H6
Conjugation	Unconjugated

Storage & Handling

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Storage	Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B7 homolog 6; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167; natural cytotoxicity triggering receptor 3 ligand 1; DKFZp686O24166; B7H6; B7-H6

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Rabbit anti-B7-H6 Recombinant Monoclonal Antibody [BLR271L]

Detection of human B7-H6 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, K-562, Hep-G2, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human B7-H6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used for IP at 6 µl/mg lysate

Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of Hep-G2 cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of HEK293T cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.