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Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]

SKU: orb1519961

Description

CD247/CD3Z Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

Tested ApplicationsFC, IHC, IP, SW-Size, WB
Dilution RangeWB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ReactivityHuman
Application Notes
Format: Whole IgG

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
ImmunogenBetween 114 and C-terminus
TargetCD247/CD3Z
ConjugationUnconjugated

Storage & Handling

Storage2 - 8°C
Form/AppearanceWhole IgG
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration100 µg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD3Z antigen, zeta polypeptide (TiT3 complex); CD antigen CD247; TCR zeta chain; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T3Z; IMD25; CD3-ZETA; TCRZ; CD3Z; CD3Q; CD3H; CD247 antigen, zeta subunit; CD3zeta chain

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Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]

Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (orb1519961) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
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Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2] (orb1519961)

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Available Sizes

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100 μl
$ 670.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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