Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]

SKU: orb1519964

This product can be custom made

Description

CD3E Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

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Item 1 of 1

Tested Applications	FC, IF, IHC, IP, WB
Dilution Range	WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Immunogen	Between 157 and C-terminus
Target	CD3E
Conjugation	Unconjugated

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

1 Results

Dilution

Sample

Summary

IHC

Human

1:250

Ormalin-fixed paraffin-embedded (FFPE) normal intestinal tissue and immune-related enterocolitis (irEC) tissue biopsies

The antibody was used on human...

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	50 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T3E

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]

Detection of human CD3E (orange) , CD8 alpha (red) , and PD-L1 (green) in FFPE breast carcinoma by IHC-IF. Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (orb1519964) , rabbit anti-CD8 alpha recombinant monoclonal, rabbit anti-PD-L1 recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766

RefSeq (Protein):NP_000724.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000724.1

Documents Download

Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence

Immunoprecipitation