Rabbit anti-CD70 Recombinant Monoclonal Antibody [BLR266L]

SKU: orb1784579

Description

Rabbit anti-CD70 Recombinant Monoclonal Antibody [BLR266L]

Research Area

,Primary Antibodies

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Immunogen: Residues 50-193 (ECD)
• Target: CD70
• Conjugation: Unconjugated

Storage & Handling

• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor ligand 8A; TNFSF7; Ki-24 antigen; CD27LG; CD27-L; CD27L; CD27 ligand; CD antigen CD70; TNLG8A; CD70 antigen

Detection of human CD70 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, 786-O (10 µg) , HEK293T, THP-1, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784579) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD70 by immunohistochemistry. Sample: FFPE section of human lymph node with metastatic lung cancer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784579). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD70 by immunocytochemistry. Sample: FFPE section of 786-O cells. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784579). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CD70

UniProt

P32970