Rabbit anti-CTLA4 Recombinant Monoclonal Antibody [BLR257L]

SKU: orb1784582

Description

Rabbit anti-CTLA4 Recombinant Monoclonal Antibody [BLR257L]

Research Area

,Primary Antibodies

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Immunogen: Between 173 and C-terminus
• Target: CTLA4
• Conjugation: Unconjugated

Storage & Handling

• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GSE; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; IDDM12; GRD4; cytotoxic T-lymphocyte-associated serine esterase-4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte protein 4; cytotoxic T lymphocyte associated antigen 4 short spliced form; CELIAC3; celiac disease 3; CD152 isoform; CD152; CD antigen CD152; CD; ALPS5; CTLA-4; insulin-dependent diabetes mellitus 12

Detection of human CTLA4 by western blot. Samples: Whole cell lysate (50 µg) from A2058, NCI-H520, MJ with 10ng/ml PMA + 500ng/ml Ionomycin for 24hrs, My-La CD4+, and NK-92 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784582) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CTLA4 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784582). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTLA4 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784582). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTLA4 (magenta) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784582) used at 1:20. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

CTLA4

UniProt

P16410