Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody [BLR295L]

SKU: orb1806427

This product can be custom made

Description

Research Area

,Primary Antibodies

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IHC, IP, WB
Dilution Range	WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Immunogen	Residues 22-555 (ECD)
Target	E-Selectin/CD62E
Conjugation	Unconjugated

Storage & Handling

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Storage	Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; leukocyte endothelial cell adhesion molecule 2; LECAM2; ESEL; CD antigen CD62E; ELAM1; ELAM; CD62E; CD62 antigen-like family member E; E-selectin; ELAM-1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody [BLR295L]

Detection of human E-Selectin/CD62E by western blot. Samples: Whole cell lysate (50 µg) from HUVEC-C cells untreated (-) or treated (+) with 10ng/mL TNFalpha. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806427) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human E-Selectin/CD62E (shaded) in TNF-alpha treated HUVEC cells by flow cytometry. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806427) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HUVEC-C cells treated with 10ng/mL TNFalpha. Antibodies: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806427) used for IP at 6 µl/mg lysate.

Detection of human E-Selectin/CD62E by immunocytochemistry. Sample: FFPE section of HUVEC-C cells treated with TNF-alpha (left) and mock treated HUVEC-C cells (right). Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806427). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806427). Secondary: HRP-conjugated goat anti-rabbit IgG.