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Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]
SKU: orb1519999
Description
Research Area
Product Categories/Products/Antibodies,Primary Antibodies
Images & Validation
−![Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]](/images/quality_badge_antibodies.png)
| Tested Applications | ChIP, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections. Overnight incubations are suggested.; ICC - 1:200 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Immunogen | Between 400 and 450 |
| Target | HIF2-alpha |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 400 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ECYT4; HIF2-alpha; PAS domain-containing protein 2; MOP2; member of PAS protein 2; Hypoxia-inducible factor 2-alpha; PASD2; hypoxia-inducible factor 2 alpha; HLF; HIF-2-alpha; HIF2A; HIF-1alpha-like factor; HIF-1-alpha-like factor; endothelial PAS domain-containing protein 1; class E basic helix-loop-helix protein 73; bHLHe73; basic-helix-loop-helix-PAS protein MOP2; EPAS-1
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001421.2 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation