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Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L]
Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:1,000, IP: 15 µl/1 mg lysate, IHC: 1:1000 to 1:4000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Immunogen | Residues 27-436 (ECD) |
| Target | Interferon alpha/beta receptor 1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 400 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L] [orb1710814]
FC, IHC, IP, WB
Human
Rabbit
Recombinant
Unconjugated
10 μlRabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L] [orb1710815]
IHC, IP, WB
Human
Rabbit
Recombinant
Unconjugated
100 μg

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![Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L]](/images/pub/media/catalog/product/NewWebsite/11/orb1710816_wb_1.jpg)
Detection of human Interferon alpha/beta receptor 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, RPMI-8226, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710816) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
![Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L]](/images/pub/media/catalog/product/NewWebsite/11/orb1710816_ihc_1.jpg)
Detection of human Interferon alpha/beta receptor 1 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710816). Secondary: HRP-conjugated goat anti-rabbit IgG.
![Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L]](/images/pub/media/catalog/product/NewWebsite/11/orb1710816_flow-cyt_1.jpg)
Detection of human Interferon alpha/beta receptor 1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
![Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L]](/images/pub/media/catalog/product/NewWebsite/11/orb1710816_ip_1.jpg)
Detection of human Interferon alpha/beta receptor 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710816) used for IP at 15 µl/mg lysate.
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Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody [BLR265L] (orb1710816)
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