Rabbit anti-NEDD4L Antibody, Affinity Purified

SKU: orb1526488

Description

NEDD4L Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 300 and 350
• Target: NEDD4L
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase NEDD4-like; PVNH7; RSP5; ubiquitin-protein ligase Rsp5; NEDD4.2; HECT-type E3 ubiquitin transferase NED4L; hNEDD4-2; NEDD4-2

Detection of human and mouse NEDD4L by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NEDD4L antibody orb1526488 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. NEDD4L was also immunoprecipitated by rabbit anti-NEDD4L antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 second (A) and 3 seconds (B).

Detection of human NEDD4L by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NEDD4L (Cat. No. orb1526488) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NEDD4L

UniProt

Q96PU5

RefSeq (Protein):NP_001138439.1