Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody [BLR267L]

SKU: orb1784576

This product can be custom made

Description

Research Area

,Primary Antibodies

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Immunogen	Residues 42-771 (ECD)
Target	P-Selectin/CD62P
Conjugation	Unconjugated

Storage & Handling

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Storage	Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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LECAM3; leukocyte-endothelial cell adhesion molecule 3; PADGEM; platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; P-selectin; GRMP; PSEL; granule membrane protein 140kDa; granule membrane protein 140; GMP-140; GMP140; CD62P; CD62 antigen-like family member P; CD antigen CD62P; CD62; granulocyte membrane protein; selectin P (granule membrane protein 140kDa, antigen CD62)

Quality Guarantee

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Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody [BLR267L]

Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (red) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).