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Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H]
Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IP - 20 µl/1 mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Immunogen | Surrounding tyrosine 15 |
| Target | CDK1, Phospho (Y15) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 40 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H] [orb1519744]
IP, WB
Human
Rabbit
Recombinant
Unconjugated
100 μlRabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H] [orb1976074]
IP, WB
Human
Rabbit
Recombinant
Unconjugated
100 μg

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Detection of human Phospho CDK1 (Y15) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated (+) with Hydroxyurea or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonal antibody (orb1519743) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001777.1 |
|---|
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Protocol Information
Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H] (orb1519743)
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